SEROPREVALENCE OF BOVINE AND CAPRINE BRUCELLOSIS DETECTED BY MILK RING TEST AND INDIRECT ELISA AT HYDERABAD, SINDH, PAKISTAN

Om Parksah Suthar; Imam Bakhsh; Rameez Raja Kaleri; Tashkain Lashari; Abdullah Shaikh; Kamal- Uddin Mandokhail; Sakhi Bakhsh; Deepesh Kumar Bhuptani; Muhammad Ishaq; Muhammad Anees Memon

doi:10.71146/kjmr868

Authors

Om Parksah Suthar Veterinary Research and Diagnosis (CVDL), Sindh, Tando Jam. Author
Imam Bakhsh Livestock and Dairy Development Department, Quetta, Balochistan. Author
Rameez Raja Kaleri Veterinary Research and Diagnosis (CVDL), Sindh, Tando Jam. Author
Tashkain Lashari Faculty of Animal Husbandry and Veterinary Sciences, Sindh Agriculture University, Tando Jam. Author
Abdullah Shaikh Faculty of Animal Husbandry and Veterinary Sciences, Sindh Agriculture University, Tando Jam. Author
Kamal- Uddin Mandokhail Livestock and Dairy Development Department, Quetta, Balochistan. Author
Sakhi Bakhsh Livestock and Dairy Development Department, Quetta, Balochistan. Author
Deepesh Kumar Bhuptani Department of Meat Technology, SBBUVAS, Sakrand. Author
Muhammad Ishaq Department of Poultry Husbandry, Sindh Agriculture University, Tando Jam. Author
Muhammad Anees Memon Department of Veterinary Physiology and Biochemistry, SBBUVAS, Sakrand. Author

DOI:

https://doi.org/10.71146/kjmr868

Keywords:

Brucellosis, Brucella abortus, Brucella melitensis, Milk Ring Test, Indirect ELISA, Seroprevalence, Abortion, Buffaloes, Goats, Hyderabad Sindh

Abstract

Brucellosis is a major zoonotic disease that significantly affects livestock productivity and poses a serious threat to public health, particularly in developing countries where dairy farming is a primary source of income. The present study was conducted at the Veterinary Research and Diagnosis Laboratory (CVDL), Tando Jam, to determine the seroprevalence of brucellosis in buffaloes and goats of Hyderabad district, Sindh, Pakistan. A total of 420 milk samples were collected from apparently healthy and clinically suspected animals, including buffaloes (n = 240) and goats (n = 180), and screened using Milk Ring Test (MRT) and Milk indirect ELISA (i-ELISA) for the detection of anti-Brucella antibodies. The overall prevalence detected by MRT was 6.9% (29/420), while i-ELISA detected a higher prevalence of 12.4% (52/420), indicating greater sensitivity of the ELISA technique. Species-wise analysis showed comparatively higher seropositivity in buffaloes than goats. Statistical analysis revealed a highly significant association (P < 0.01) between abortion history and brucellosis seropositivity, whereas stillbirth showed no significant association. The findings confirm the active circulation of Brucella infection in the study area and emphasize the need for routine screening, improved biosecurity measures, and implementation of effective control and vaccination strategies.

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